Analytical Techniques In DNA Sequencing

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Brian K. Nunnally (Wyeth Vaccines, Sanford, North Carolina, USA) Brian K. Nunnally (Wyeth Vaccines, Sanford, North Carolina, USA)

Analytical Techniques In DNA Sequencing

Brian K. Nunnally (Wyeth Vaccines, Sanford, North Carolina, USA), Brian K. Nunnally (Wyeth Vaccines, Sanford, North Carolina, USA), Edward S. Yeung (Iowa State University, Iowa, USA) , Victor W. Weedn (Duquesne University, School of Law, Pennsylvania, USA Duquesne University Duquesne University, PIttsburgh, Pennsylvania, USA Duquesne University, USA)

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English

CRC Press Inc
23 June 2005

Cytology; Biochemistry; Molecular biology

Since the early days of radioisotope sequencing, a wide variety of new DNA sequencing techniques have emerged. New methods are aimed at reducing the amount of sample needed, improving the accuracy, and reducing the amount of time needed to generate a sequence. This text discusses the different analytical DNA sequencing techniques, as well as exciting applications of DNA sequencing. Advances in DNA separations using advanced capillary electrophoresis and microchip sequencing are presented. Chapters cover improvements in the detection of DNA sequencing fragments, including types of fluorescence detection, the use of mass spectrometry, and single molecule detection. The uses of DNA sequencing in forensics, the sequencing of ancient DNA, and genome sequencing are also included.

Contributions by: Edward S. Yeung (Iowa State University Iowa USA), Victor W. Weedn (Duquesne University, School of Law, Pennsylvania, USA Duquesne University Duquesne University, PIttsburgh, Pennsylvania, USA Duquesne University, USA), Markus Sauer (Physikalisch-Chemisches Institut, Heidelberg, Germany)
Edited by: Brian K. Nunnally (Wyeth Vaccines Sanford North Carolina USA), Brian K. Nunnally (Wyeth Vaccines, Sanford, North Carolina, USA)
Imprint: CRC Press Inc
Country of Publication: United States
Dimensions: Height: 234mm, Width: 156mm, Spine: 20mm
Weight: 498g
ISBN: 9780824753429
ISBN 10: 0824753429
Pages: 256
Publication Date: 23 June 2005
Audience: Professional and scholarly , Professional and scholarly , Professional & Vocational , Undergraduate , Further / Higher Education
Format: Hardback
Publisher's Status: Active

Introduction to DNA Sequencing: Sanger and Beyond. Developments in the Detection of DNA Sequencing Fragments Using Fluorescence: Energy Transfer and Fluorescence Lifetimes. Microscale Sample Preparation for DNA Sequencing and Genotyping. Microfabricated DNA Sequencing Devices. Analysis of Nucleic Acids by Mass Spectrometry. Sequencing the Single DNA Molecule. DNA Sequencing for Genome Analysis. Sequence Reconstruction from Nucleic-Acid Micro-Array Data. Ancient DNA. Forensic DNA Sequencing.

Brian K. Nunnally