Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
Volume editor:
Grant Jensen
Imprint: Academic Press Inc
Country of Publication: United States
Edition: 481st edition
Volume: 481
Dimensions:
Height: 229mm,
Width: 152mm,
Spine: 28mm
Weight: 880g
ISBN: 9780123749062
ISBN 10: 0123749069
Series: Methods in Enzymology
Pages: 464
Publication Date: 14 October 2010
Audience:
Professional and scholarly
,
Undergraduate
Format: Hardback
Publisher's Status: Active
Historical perspective 3D reconstruction from electron micrographs: a personal account of its development David DeRosier Preparation of 2-D crystals Henning Stahlberg Helical Crystallization of Soluble and Membrane Binding Proteins Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie Plunge Freezing for Electron Cryomicroscopy Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen A Practical Guide to the Use of Monolayer Purification and Affinity Grids Thomas Walz, Deborah F. Kelly, Danijela Dukovski GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM Holger Stark Cryo-negative staining of macromolecular assemblies Sacha De Carlo, Holger Stark Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM Fred J. Sigworth, Liguo Wang Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing Mark S. Ladinsky Site-Specific Biomolecule Labeling with Gold Clusters Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld How to Operate a Cryo-Electron Microscope Huilin Li, Jingchuan Sun Collecting electron crystallographic data of two-dimensional protein crystals Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng Automated Data Collection for Electron Microscopic Tomography David Agard, Shawn Q. Zheng, J. W. Sedat Correlated Light and Electron Cryomicroscopy Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz Phase Plates for Transmission Electron Microscopy Radostin Danev, Kuniaki Nagayama Radiation damage in electron cryomicroscopy John L. Rubinstein, Lindsay A. Baker
